MoP4 - Master of Pores 4

Master of Pores is a pipeline written in Nextflow DSL2 to analyze Nanopore data.

It can handle reads from direct RNAseq, cDNAseq, DNAseq etc.

The name is inspired by the Metallica's Master Of Puppets, the logo and the images are an homage to Akira Toriyama

Install

Please install nextflow and singularity or docker before.

Then download the repo:

git clone --recurse-submodules https://github.com/biocorecrg/master_of_pores.git

Documentation

The documentation is available at https://biocorecrg.github.io/master_of_pores/MOP4-dev/

Contact

Please open an issue if you encounter any issues / troubles. However, please go over the previous issues (including closed issues) before opening a new issue, as your same exact question might have been already answered previously. Thank you!

Reference

If you use this tool, please cite our papers:

"Nanopore Direct RNA Sequencing Data Processing and Analysis Using MasterOfPores" Cozzuto L, Delgado-Tejedor A, Hermoso Pulido T, Novoa EM, Ponomarenko J. N. Methods Mol Biol. 2023;2624:185-205. doi: 10.1007/978-1-0716-2962-8_13.

"MasterOfPores: A Workflow for the Analysis of Oxford Nanopore Direct RNA Sequencing Datasets" Luca Cozzuto, Huanle Liu, Leszek P. Pryszcz, Toni Hermoso Pulido, Anna Delgado-Tejedor, Julia Ponomarenko, Eva Maria Novoa. Front. Genet., 17 March 2020. https://doi.org/10.3389/fgene.2020.00211