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Overview

Before the event

Tech setup

In addition to the actual workshop, we offer two pre-workshop sessions designed to help participants with any issues installing software required for the workshop. During these, we also encourage participants to ask questions about basic Python/Jupyter Notebook usages (the notebook discussed in these sessions can be found here). The two sessions take place on:

  • Wednesday (02.04.2025), 15:00 - 17:00 CET
  • Thursday (03.04.2025), 15:30 - 17:30 CET

The Zoom links to these events can be found in the mail sent on Sunday, 30.03.2025. If you have any other questions, please direct them to the 2025-04: Workshop GSCN > pre-workshop support Zulip channel.

Syllabus

Note: Schedule subject to minor adjustments.

Date Time Activity
Monday, 07.04.2025 09:00 – 09:15 Welcome & Introduction to the Workshop
09:15 – 10:00 Talk: "An in-depth look at AnnData" by Ilan Gold
10:00 – 11:30 1) Basic pre-processing of scRNA-seq data
2) Batch effect correction
3) Downstream analysis
11:30 - 12:00 Hands-on coding & Troubleshooting
12:00 – 13:00 Lunch
13:00 – 13:30 Intro to "Trajectory analysis"
13:30 – 13:40 Break
13:40 – 15:20 Trajectory analysis
15:20 – 15:40 Break
15:40 – 17:30 Trajectory analysis
19:00 - tbd Dinner at Porta Nova (Robert-Koch-Platz 12)
Tuesday, 08.04.2025 09:00 – 09:30 Recap and Q&A, tech setup for spatial workshop
09:30 – 10:00 Talk: "Clonal dynamics and mitochondrial genetics through the
lens of single-cell multi-omics" by Leif Ludwig
10:00 – 11:30 1) Ligand-receptor interaction analysis
2) Differential cellular abundance
11:30 - 12:00 Hands-on coding & Troubleshooting
12:00 – 13:00 Lunch
13:00 – 13:30 Intro to "Spatial Omics"
13:30 – 13:40 Break
13:40 – 15:20 Spatial preprocessing & analysis
15:20 – 15:40 Break
15:40 – 17:30 Spatial preprocessing & analysis
20:00 - tbd Dinner (self-organised)
Wednesday, 09.042025 09:00 – 09:30 Talk: “Spatiotemporal dynamics of tumor development in a novel
triple-negative breast cancer model" by Tancredi Massimo Pentimalli
09:30 – 11:30 Talk: "Foxa2 pioneers enteroendocrine lineage recruitment from
intestinal stem cells" by Michael Sterr
10:00 – 11:30 Option A) Reproduce published figures
Option B) Discuss your own data/analysis
11:30 – 12:00 Results / Feedback

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